
Note: For example, 1 μl of 1000X stock solution into 200 μl of Maintenance Medium in the 96-well plate yields a 5X intermediate solution with 0.5% DMSO. iCell Cardiomyocytes2 can tolerate 0.1-0.5% …
How do I prepare and store dexamethasone used in the hepatocyte …
For use with iCell ® Hepatocytes 2.0, you can prepare a 5 mM stock solution of dexamethasone in DMSO, vortex to mix, and then dispense and store aliquots at -20°C for up to 1 year.
When can I assay or perform gene expression analysis on your ...
iCell Cardiomyocytes have been cultured for >120 days (up to 9 months). For more details about how long cardiomyocytes can be maintained in culture, see Solution 00000019B. Reference:
What are the storage recommendations and stability of poly-L …
The World Leader in Stem Cell TechnologyWhat are the storage recommendations and stability of poly-L-ornithine (PLO) and laminin coated plates used with GABANeurons?
How do you recommend culturing iCell DopaNeurons for a …
Add sodium metabisulfite from a 1000x stock solution to a final concentration of 4 mM. Immediately run ELISA assay or store supernatant with preservatives in –80°C for up to 2 weeks.
Are there other ECMs beside gelatin that I can use for plating iCell ...
While our standard recommendation for plating iCell® Cardiomyocytes is to use 0.1% gelatin (see solution 00000082A), we have tested other common ECMs, including fibronectin and collagen, (with …
What is the part (catalog) number for your hepatocyte kit?
The part (catalog) number for the iCell<sup>®</sup> Hepatocytes 2.0 Kit is R1027. For more details about our hepatocyte kit contents, see Solution 00000202D, below.
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Do you have a protocol for BacMam/AAV/Lenti gene delivery in iCell ...
Try a 10% virus load by volume (v/v) with and without Enhancer Solution (0.2X final concentration, diluted from a 1 mM (1000X) stock solution). On day 9, remove the BacMam transduction solution.
What osmolarity and pH values work for patch clamping cardiomyocytes ...
The intracellular saline solution should have an osmolarity of approximately 310 mOsm and a pH of approximately 7.2. Glucose can be added to the solution to increase osmolarity.